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Briefly, slides were immersed in 4% paraformaldehyde (Thermo Fisher Scientific, catalog: J19943.K2) immediately after removal from the freezer for 1 min, then washed with water

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Notably, reduced melatonin levels and disrupted 24-hour melatonin rhythms are frequently observed in individuals with AD dementia [36,37,38,39,40]

Alcohol Res G=Health 27:277284 Xu B, Cui Y, Wang W, Li S (2020) Immunomodulation-enhanced nanozyme-based tumor catalytic therapy

Step 9: Visually Inspect the Solution Hold the vial up to a light source and examine the solution before proceeding: Step 10: Aliquot and Label If you will not use the entire reconstituted volume within one experiment session, aliquot into single-use volumes before storage